STANDARD TUBE (ST) METHOD INSTEAD OF TISSUE CULTURE PLATE (TCP) METHOD FOR DETECTION OF BIOFILM PRODUCTION AMONG COAGULASE NEGATIVE STAPHYLOCOCCI (CONS) ISOLATES IN A RURAL TERTIARY CARE HOSPITAL

Dr. Abhishek Debnath, Reshmi Ghosh, Dr. Dipayan Ghosh

Abstract


Introduction: The two main reasons of the increasing rate of CONS infections in recent years are the spreading of antibiotic resistance and increasing use of medical devices. Because of bioflim formation on medical devices, maximum of hospital acquired infections are caused by CONS. Biofilm formation also increases the resistance to antimicrobial agents and host defense mechanisms and because of that, it is very difficult to eradicate biofilm associated infections by conventional antibiotic treatment. Hence, the present study was undertaken with following aims and objectives.

Aims and Objectives: To study biofilm production among the isolated species of CONS by Tissue culture plate (TCP) method and Standard tube method (ST) and to compare these methods for biofilm production.

Materials and Methods:

180 CONS strains isolated from clinically significant samples were identified by different conventional methods and biofilm production was detected by TCP and ST method.

Results and Discussion:

Among 180 CONS isolates, predominant isolated species were S. epidermidis (40.56%), S.haemolyticus (25%). 60% and 54.44% CONS isolates were positive for biofilm production by TCP and ST method. Comparison of ST method with TCP method by Pearson chi square test showed strong association between TCP and ST and this distribution is statistically significant with P value of 0.006.

Conclusion: ST method can be used in routine hospital laboratories for detection of biofilm production as it shows reliable results with good sensitivity and specificity.


Keywords


Coagulase negative staphylococci (CONS), Tissue culture plate (TCP) method.

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References


[I] Badampudi V, S.S, Surya Kirani K.R.L, Gunti R (2016). Speciation and biofilm production of coagulase negative Staphylococcal isolates from clinically significant specimens and their antibiogram. JKIMSU; 5(2): 69-78.

[II] Colle JG, Miles RS, Watt B (2008). Mackie and McCartney, practical medical microbiology, 14 th ed. New Delhi: Indian Reprint: 131-149.

[III] Davenport DS, Massanari RM, Pfaller MA, et al. (1986) Usefulness of a test for slime production as a marker for clinically significant infections with coagulase-negative staphylococci. J Infect Dis. 153(2): 332 – 339.

[IV] Deka N (2014). Comparison of Tissue Culture plate method, Tube Method and Congo Red Agar Method for the detection of biofilm formation by Coagulase Negative Staphylococcus isolated from Non-clinical Isolates. Int. J. Curr. Microbiol. App. Sci. 3(10): 810-815.

[V] Devapriya F, Sajith P, Ranganathan R, Shanmugam J (2014). Prevalence of biofilm and beta-lactamase producing Staphylococcus in nasal and throat isolates from healthy volunteers: A medical alert. NJMS. 3 (2): 79-83.

[VI] Lucía E. Alcaráz, Sara E. Satorres, Rodolfo M. Lucero, Olga N. Puig de Centorbi (2003). Species identification, slime production and oxacillin susceptibility in coagulase-negative staphylococci isolated from nosocomial specimens. Braz. J. Microbiol. 34(1):45-51.

[VII] Otto, M (2004). Virulence factors of the Coagulase negative staphylococci. Front. Biosci. 9: 841-863.

[VIII] Puneet Bhatt Maj, Kundan Tandel Capt, Singh MA, M. Mugunthan, Col Naveen Grover, A.K. Sahni Brig (2016). Species distribution and antimicrobial resistance pattern of Coagulase-negative Staphylococci at a tertiary care centre. Med J Armed Forces India. 72 (1) 71–74.

[IX] Rupp, M.E. and Archer, G.L(1994). Coagulase negative Staphylococci: pathogens associated with medical progress. Clin. Infect. Dis. 19(2):231-243.

[X] Sadhvi Parashar (2014). Significance of Coagulase Negative Staphylococci with Special Reference to Species Differentiation and Antibiogram. Indian Med Gaz.; 255-258.

[XI] Singh S, Asthana A. K, Madan M, Ghosh S. J (2015). Study of Biofilm Formation Among Clinical Staphylococcal Isolates. NJLM. 4(4): 24-27.

[XII] Thilakavathy P, Priyan RM, Jagatheeswari PA, Charles J, Dhanalakshmi V, Lallitha S, Rajendran T, Divya B (2015). Evaluation of Ica Gene in Comparison with Phenotypic Methods for Detection of Biofilm Production by Coagulase Negative Staphylococci in a Tertiary Care Hospital. J Clin Diagn Res. 9(8):DC16-19.

[XIII] Washington CW Jr, Stephen DA, William MJ et al. (2006) Koneman’s color Atlas and Textbook of diagnostic Microbiology. 6th ed.USA: Lippincott Williams and Williams; 623-71.


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