Anju Viswan K., E. Pushapalatha


Usage of photochemical may serve as suitable alternative to synthetic insecticides, which causes various environmental consequences and resulted in development of resistance in insects. The larvicidal activity of leaf extracts of Adhatoda vasica, Centella asiatica, Holarrhena antidysentrica, Hugonia mystax, Mentha spicata on 3rd instra larvae of Aedes agypti L. and Culex quinquefacsiatus Say were determined in the resent study. Methanol leaf extracts were used for the study and after 24 hours mortality were recorded and compared to find out the most active plant extracts. M.spicata leaf extract had shown more activity as compared with other plant extracts. The phytochemical screening was also done to find out the active compounds present in the methanolic extracts of M.spicata. The findings of the present study suggest that further purification and isolation of active principle could lead development of more potent and ecofriendly biocontrol agents against mosquitoes.


Larvicidal activity, Aedes aegypti, Culex quinquefasciatus, Adhatoda vasica, Centella asiatica, Holarrhena antidysentrica, Hugonia mystax, Mentha spicata

Full Text:



Abbot, W.S. (1925). A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18(2), 265-267.

Anju Viswan, K. and Pushpalatha, E. (2019). Phytochemical Screening and Larvicidal Activity of Croton hirtus L’Her Leaves against Culex quinquefasciatus Say. International journal of Science and Research, 8(8), 878-880.

Ansari, M.A. and Razdan, R.K.(1995). Relative efficacy of various oils in repelling mosquitoes. Indian Journal of Malariology, 32, 104-111.

Atul Kumar Gangwar and Ashoke K. Ghosh. (2014). Medicinal uses and Pharmacological activity of Adhatoda vasica. International Journal of Herbal Medicine, 2 (1), 88-91.

Bhaskaran,V. And Narayanasamy, P. (1995). Traditional pest control. Caterpillar publications. Mariyappa Nagar, Tamil Nadu, India.

D. J. Finney, “Probit Analysis”, Cambridge University Press, 1971.

Dharmesh, K.P. and Natvarlal, M.P. (2010). Pharmacognostic and Phytochemical Investigations of the Leaves of Tecoma Stans Linn. International Journal of Pharmacology Science Review and Reserach, 3(1), 70-72.

Edeogo, H.O., Okwn, D.F. and Mbaebie, B.O. (2005). Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology, 4(7), 685-688.

Jang, Y. S., Baek, B. R., Yang, Y. C., Kim, M. K. and Lee, H.S. (2002). Larvicidal activity of leguminous seeds and grains against Aedes aegypti and Culex pipiens pallens. Journal of the American Mosquito Control Association, 18(3), 210-213.

Knio, K. M., Usta, J., Dagher, S., Zournajian, H. and Kreydiyyeh, S. (2008). Larvicidal activity of essential oils extracted from commonly used herbs in Lebanon against the seaside mosquito, Ochlerotatus caspius. Bioresource Technology, 99(4), 763-768.

Marimuthu Govindarajan, Sivakumar, R., Rajeswari, M. and Yogalakshmi, K. (2012). Chemical composition and larvicidal activity of essential oil from Mentha spicata (Linn.) against three mosquito species. Parasitology research, 110(5); 30-32.

Marimuthu Govindarajan., Kadaikunnan, S., Naiyf, S. Alhabi. And Giovanni Benelli. (2017). Single-step biological fabrication of colloidal silver nanoparticles using Hugonia mystax: larvicidal potential against Zika virus, dengue, and malaria vector mosquitoes. Artificial Cells, Nanomedicine, and Biotechnology, 45(7), 1317-25.

Modupe Elizabeth Ojewumi., Samuel Oluwafunsho Adedokun., Oladele Julius Omodara., Esther Adenike Oyeniyi., Olugbenga Samson Taiwo. and Emmanuel Omotayo Ojewumi. (2017). Phytochemical and Antimicrobial Activities of the Leaf Oil Extract of Mentha Spicata and its Efficacy in Repelling Mosquito International Journal of Pharmaceutical Research and Allied Science, 6(4):17-27.

Muthukrishnan, J. and Pushpalatha, E. (2008). Effects of plant extracts on fecundity and fertility of mosquitoes. Journal of Applied Entomology, 125(1–2), 31–35.

Muthukrishnan, J., Pushpalatha, E. and Kasthuribhai, A. (1997). Biological Effects of Four Plant Extracts on Culex quinquefasciatus Say Larval Stages. International Journal of Tropical Insect Science, 17(3–4), 389–394.

Pandian, R. S., Abraham, M. G. and Manoharan, A. C. (1994). Susceptibility of the larvae of Culex quinquefasciatus Say to extracts of medicinal plants. Environmetal Pollution, 1(3–4), 109–112.

Pushpalatha, E. and Anju Viswan, K. (2013). Adulticidal and repellent activities of Melaleuca leucadendron (L.) and Callistemon citrinus (Curtis) against filarial and dengue vectors. Entomon, 38(3), 149–154.

Rajkumar, S. And Jabanesa, A. (2004). Mosquito larvicidal activities of octacosane from Mochoma polystachym (Lameaceae). Journal of Ethnopharmocology, 90(1): 87-89.

Raman, M.S., Nasim Suttana, M.N. and Anwar. (2004). Invitro antimicrobial activity of holariffine-24ol isolated from the stem bark of Hollarhena antidysentrica. International journal of Agriculture Biology, 6(4): 698-700

Sadek, M.M. (2003). Antifeedant and toxic activity of Adhatoda vasica leaf extract against Spodoptera littoralis. Applied Entomology, 127:396-404.

Samidurai, K., Jebanesan, A., Saravanakumar, A., Govindarajan, M. and Pushpanathan, T. (2009). Larvicidal, ovicidal and repellent activities of Pemphis acidula Forst. (Lythraceae) against filarial and dengue vector mosquitoes. Academic Journal of Entomology, 2(2):62-66.

Service, M.W. (1983). Management of vector, in Biological control of mosquitoes- has it a future. Mosquito News, 43: 113-120.

Tamilselvan, P., Chinnasamy, R. and Devarajan, N. (2015). Larvicidal, pupicidal and adulticidal potential of Ocimum gratissimum plant leaf extracts against filariasis inducing vector. International Journal of Mosquito Research, 2 (2), 01-08.

Trease, G. E. and Evans, W. C. ( 1989). “Pharmacognsy”. 11th edn. Brailliar Tridel Can. Macmillan Publishers Trease, Alden Press. Oxford, 213-232.

World Health Organization. (1989). Geographical distribution of arthropod-borne diseases and their principal vectors. WHO/VBC/89.967. Geneva, 426.


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright © 2019 International Educational Applied Scientific Research Journal